Title:Glutathionylation of the alpha-subunit of Na,K-ATPase from rat heart by oxidized glutathione inhibits the enzyme.
Authors:Xianyu M; Petrushanko IY; Klimanova EA; Dergousova EA; Lopina OD
Publication:Biochemistry (Mosc). 2014 Feb;79(2):158-64. doi: 10.1134/S0006297914020096.
A partially purified Na,K-ATPase preparation from rat heart containing alpha1- and alpha2-isoforms of the enzyme was shown to include both subunits in S-glutathionylated state. Glutathionylation of the alpha1-subunit (but not of the alpha2-subunit) was partially removed when the preparation was isolated in the presence of dithiothreitol. The addition of oxidized glutathione irreversibly inhibited both isoforms. Inhibition of the enzyme containing the alpha1-subunit was biphasic, and the rate constants of the inhibition were 3745 +/- 360 and 246 +/- 18 M(-1).min(-1). ATP, ADP, and AMP protected the Na,K-ATPase against inactivation by oxidized glutathione.