Title:Molecular identification of the enzyme responsible for the mitochondrial NADH-supported ammonium-dependent hydrogen peroxide production.
Authors:Kareyeva AV; Grivennikova VG; Cecchini G; Vinogradov AD
Publication:FEBS Lett. 2011 Jan 21;585(2):385-9. doi: 10.1016/j.febslet.2010.12.019. Epub 2010 Dec 17.
A homogeneous protein with a subunit apparent molecular mass of approximately 50 kDa that catalyzes the previously described mitochondrial NADH-supported ammonium-stimulated hydrogen peroxide production (Grivennikova, V.G., Gecchini, G. and Vinogradov, A.D. (2008) FEBS Lett. 583, 1287-1291) was purified from the mitochondrial matrix of bovine heart. Chromatography of partially purified protein showed that the peaks of ammonium-stimulated NADH-dependent H(2)O(2) production and that of NADH:lipoamide oxidoreductase activity coincided. The catalytic properties and mass spectrometry of the trypsin-digested protein revealed peptides that allowed identification of the protein as the Bos taurus dihydrolipoyl dehydrogenase.