Title:Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.
Authors:Tamm NN; Semenov AG; Seferian KR; Bereznikova AV; Murakami MM; Apple FS; Koshkina EV; Krasnoselsky MI; Katrukha AG
Publication:Clin Biochem. 2011 Feb;44(2-3):257-9. doi: 10.1016/j.clinbiochem.2010.09.030. Epub 2010 Oct 13.
OBJECTIVES: To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays. DESIGN AND METHODS: Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli). RESULTS: The SES assay measured 1.2- to 7.2-fold (2.1+/-0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference. CONCLUSIONS: The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.