Article

Title:Ammonium-dependent hydrogen peroxide production by mitochondria.
Authors:Grivennikova VG; Cecchini G; Vinogradov AD
Publication:FEBS Lett. 2008 Aug 6;582(18):2719-24. doi: 10.1016/j.febslet.2008.06.054. Epub 2008 Jul 11.
PubmedID18619961
Abstract
NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (approximately 10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production.